rabbit polyclonal anti fxr2  (Proteintech)

Journal: Journal of Virology

Article Title: Identification of host dependency factors involved in SARS-CoV-2 replication organelle formation through proteomics and ultrastructural analysis

doi: 10.1128/jvi.00878-23

Figure Lengend Snippet: Antibodies

Article Snippet: Rabbit anti-FXR2 , 1:500 , , Cell Signaling Technology , Cat.#7098.

Techniques: Western Blot

Journal: Molecular Cancer Research

Article Title: TSC2 Interacts with HDLBP/Vigilin and Regulates Stress Granule Formation

doi: 10.1158/1541-7786.mcr-20-1046

Figure Lengend Snippet: Figure 3. TSC2 localizes to mammalian SGs after oxidative stress. HeLa cells were treated with 1 mmol/L NaAsO2 for 50 minutes (A) and 30 mmol/L NaAsO2 for 2 hours (B). Cells were stained for TSC2. FXR2 was used for SG detection. DAPI was used to visualize the nuclei. Arrows indicate SGs containing TSC2. Scale bars, 20 mm, 5 mm.

Article Snippet: The following antibodies were used: TSC2 (rabbit), FXR2 (rabbit), phospho-S6(S235/236) (rabbit), phospho-eIF2a(S51), Caspase 3 (rabbit), PARP (rabbit; Cell Signaling Technology), vigilin/HDLBP (rabbit), G3BP1 (rabbit), Caprin1 (rabbit; Proteintech), G3BP1 (mouse; Abcam), TSC2 (mouse; Lifespan Biosciences), b-Actin (mouse; Milipore/Sigma-Aldrich).

Techniques: Staining

Journal: Frontiers in Pharmacology

Article Title: Emodin Rescues Intrahepatic Cholestasis via Stimulating FXR/BSEP Pathway in Promoting the Canalicular Export of Accumulated Bile

doi: 10.3389/fphar.2019.00522

Figure Lengend Snippet: Effect of emodin on the expression of FXR and BSEP in normal LO2 cells. The mRNA levels of FXR (A) and BSEP (B) were detected by RT-PCR. The protein levels of FXR1, FXR2, and BSEP were measured by Western blot (C–F) . Values are the means ± SD ( n = 3, ∗ P < 0.05 compared to the control group; ∗∗ P < 0.01 compared to the control group, as determined by Student’s t -test).

Article Snippet: Rabbit anti-human FXR1 and FXR2 antibodies were, respectively, obtained from and Cell Signaling Technology (12295S/7098S, Danvers, MA, United States).

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Control

Journal: Frontiers in Pharmacology

Article Title: Emodin Rescues Intrahepatic Cholestasis via Stimulating FXR/BSEP Pathway in Promoting the Canalicular Export of Accumulated Bile

doi: 10.3389/fphar.2019.00522

Figure Lengend Snippet: Effect of emodin on the expression of FXR and BSEP in LO2 cell after guggulsterone stimulation. The mRNA levels of FXR (A) and BSEP (B) were detected by RT-PCR. The protein levels of FXR1, FXR2, and BSEP were measured by Western blot (C–F) . Values are the means ± SD ( n = 3, ∗ P < 0.05 compared to the guggulsterone group; ∗∗ P < 0.01 compared to the guggulsterone group; # P < 0.01 compared to the control group, as determined by Student’s t -test).

Article Snippet: Rabbit anti-human FXR1 and FXR2 antibodies were, respectively, obtained from and Cell Signaling Technology (12295S/7098S, Danvers, MA, United States).

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Control

Journal: Frontiers in Pharmacology

Article Title: Emodin Rescues Intrahepatic Cholestasis via Stimulating FXR/BSEP Pathway in Promoting the Canalicular Export of Accumulated Bile

doi: 10.3389/fphar.2019.00522

Figure Lengend Snippet: Effect of emodin on the expression of FXR and BSEP in LO2 cell after GW4064 stimulation. The mRNA levels of FXR (A) and BSEP (B) were detected by RT-PCR. The protein levels of FXR1, FXR2, and BSEP were measured by Western blot (C–F) . Values are the means ± SD ( n = 3, ∗ P < 0.05 compared to the GW4064 group; ∗∗ P < 0.01 compared to the GW4064 group; # P < 0.01 compared to the control group, as determined by Student’s t -test).

Article Snippet: Rabbit anti-human FXR1 and FXR2 antibodies were, respectively, obtained from and Cell Signaling Technology (12295S/7098S, Danvers, MA, United States).

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Control

Journal: Frontiers in Pharmacology

Article Title: Emodin Rescues Intrahepatic Cholestasis via Stimulating FXR/BSEP Pathway in Promoting the Canalicular Export of Accumulated Bile

doi: 10.3389/fphar.2019.00522

Figure Lengend Snippet: Effect of emodin on the expression of FXR and BSEP after FXR was knocked down by siRNA. The mRNA levels of FXR and BSEP were detected by RT-PCR (A,B) . The protein levels of FXR1, FXR2, and BSEP were measured by Western blot (C) . Values are the means ± SD ( n = 3, # P < 0.01 compared to the control group, ∗∗ P < 0.01 compared to the FXR-siRNA group; P < 0.05 compared to the FXR-siRNA group; ∗ p < 0.05 compared to the UDCA group; p < 0.05 compared to the DXM group, as determined by Student’s t -test).

Article Snippet: Rabbit anti-human FXR1 and FXR2 antibodies were, respectively, obtained from and Cell Signaling Technology (12295S/7098S, Danvers, MA, United States).

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Control

Journal: Frontiers in Pharmacology

Article Title: Emodin Rescues Intrahepatic Cholestasis via Stimulating FXR/BSEP Pathway in Promoting the Canalicular Export of Accumulated Bile

doi: 10.3389/fphar.2019.00522

Figure Lengend Snippet: The protein levels of FXR1, FXR2, and BSEP were detected by Western blotting (A,B) . Values are the means ± SD ( n = 3, # P < 0.01 compared to the control group, ∗∗ P < 0.01 compared to the lentivirus group; ∗ P < 0.05 compared to the lentivirus group; p < 0.05 compared to the UDCA group, as determined by Student’s t -test).

Article Snippet: Rabbit anti-human FXR1 and FXR2 antibodies were, respectively, obtained from and Cell Signaling Technology (12295S/7098S, Danvers, MA, United States).

Techniques: Western Blot, Control

Journal: Frontiers in Pharmacology

Article Title: Emodin Rescues Intrahepatic Cholestasis via Stimulating FXR/BSEP Pathway in Promoting the Canalicular Export of Accumulated Bile

doi: 10.3389/fphar.2019.00522

Figure Lengend Snippet: Effect of emodin on the SHP-BSEP signaling pathway in ANIT-treated rats. The mRNA levels of FXR and BSEP were measured by real-time PCR (A) . The protein levels of FXR1, FXR2, and BSEP were detected by Western blotting (B,C) . Data were expressed as the mean ± SD. n = 3. ( # P < 0.01 compared to the control group, ∗∗ P < 0.01 compared to the model group; p < 0.05 compared to the UDCA group; Δ p < 0.05 compared to the DXM group, as determined by Student’s t -test).

Article Snippet: Rabbit anti-human FXR1 and FXR2 antibodies were, respectively, obtained from and Cell Signaling Technology (12295S/7098S, Danvers, MA, United States).

Techniques: Real-time Polymerase Chain Reaction, Western Blot, Control

Journal: Frontiers in Pharmacology

Article Title: Emodin Rescues Intrahepatic Cholestasis via Stimulating FXR/BSEP Pathway in Promoting the Canalicular Export of Accumulated Bile

doi: 10.3389/fphar.2019.00522

Figure Lengend Snippet: Effects of emodin on molecules of BSEP pathway in rat primary hepatocytes. Immunofluorescence was used to detect CK-18 protein in hepatocytes, and the red fluorescence molecule Dylight488 in primary hepatocytes was observed under a fluorescence microscope (A–C) . Cytotoxicity of emodin on primary hepatocytes was determined by CCK8 assay (D) . The mRNA levels of FXR and BSEP were detected by qRT-PCR (E) . The protein levels of FXR1, FXR2, and BSEP were detected by Western blotting (F,G) . Data are shown as the mean ± SD. n = 5. ( ∗ P < 0.05 compared to the control group; ∗∗ P < 0.01 compared to the control group).

Article Snippet: Rabbit anti-human FXR1 and FXR2 antibodies were, respectively, obtained from and Cell Signaling Technology (12295S/7098S, Danvers, MA, United States).

Techniques: Immunofluorescence, Fluorescence, Microscopy, CCK-8 Assay, Quantitative RT-PCR, Western Blot, Control

Journal: Frontiers in Pharmacology

Article Title: Emodin Rescues Intrahepatic Cholestasis via Stimulating FXR/BSEP Pathway in Promoting the Canalicular Export of Accumulated Bile

doi: 10.3389/fphar.2019.00522

Figure Lengend Snippet: (1) Effect of emodin on pathological manifestation of hepatic tissue by HE staining at 400× magnification ( A : emodin 20 mg/kg group; B : emodin 40 mg/kg group; C : emodin 80 mg/kg group; D : UDCA group; E : DXM group; F : model group; G : control group). (2) Effect of emodin on BSEP expression was examined with IHC at 400× magnification ( A : emodin 20 mg/kg group; B : emodin 40 mg/kg group; C : emodin 80 mg/kg group; D : UDCA group; E : DEX group; F : model group; G : control group. Values are the means ± SD. n = 3, ## P < 0.01 compared to the control group, ∗∗ P < 0.01 compared to the model group; p < 0.01 compared to the UDCA group; ΔΔ p < 0.01 compared to the DXM group, as determined by Student’s t -test). (3) Effect of emodin on FXR1 expression was examined with IHC at 400× magnification ( A : emodin 20 mg/kg group; B : emodin 40 mg/kg group; C : emodin 80 mg/kg group; D : UDCA group; E : DXM group; F : model group; G : control group. Values are the means ± SD. n = 3, ## P < 0.01 compared to the control group, ∗∗ P < 0.01 compared to the model group; p < 0.01 compared to the UDCA group; ΔΔ p < 0.01 compared to the DXM group, as determined by Student’s t -test). (4) Effect of emodin on FXR2 expression was examined with IHC at 400× magnification ( A : emodin 20 mg/kg group; B : emodin 40 mg/kg group; C : emodin 80 mg/kg group; D : UDCA group; E : DEX group; F : model group; G : control group. Values are the means ± SD. n = 3, ## P < 0.01 compared to the control group, ∗∗ P < 0.01 compared to the model group; p < 0.01 compared to the UDCA group; ΔΔ p < 0.01 compared to the DXM group, as determined by Student’s t -test).

Article Snippet: Rabbit anti-human FXR1 and FXR2 antibodies were, respectively, obtained from and Cell Signaling Technology (12295S/7098S, Danvers, MA, United States).

Techniques: Staining, Control, Expressing